Recombinant protein expression in Escherichia coli: advances and challenges

ROSANO, G. L.; CECCARELLI, E. A.

Frontiers in Microbiology

Frontiers

Lugar: Lausanne; Año: 2014 vol. 5 p. 1 - 17

Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies. We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.