INVESTIGADORES
TALANO Melina Andrea
congresos y reuniones científicas
Título:
ESTABLISHMENT OF TRANSGENIC HAIRY ROOT CULTURES THROUGH A SUCCESSIVE TRANSFORMATION METHOD
Autor/es:
TALANO M, WEVAR-OLLER A, SOSA ALDERETE L, ERIC I, AGOSTINI E, MEDINA MI.
Lugar:
Villa Giardino, Córdoba
Reunión:
Congreso; XV JORNADAS CIENTIFICAS, Sociedad Biología de Córdoba; 2005
Institución organizadora:
Sociedad de Biología de Córdoba
Resumen:
Hairy root (HR) cultures have received special attention in the last
years because of their multiple applications. They constitute a
plant system with rapid in vitro growth without hormones and
biochemical and genetic stability. Genetic engineering has allowed
to transform plants with foreign genes to give or to improve
special metabolic features. The establishment of transgenic HR
can be obtained through a unique step of transformation, using
genetically modified A.rhizogenes, or by an unusual method with
two steps using A. tumefaciens and A. rhizogenes. The latter
technique is called successive transformation. The aim of this
work was to obtain transgenic HR cultures with peroxidases genes
(tpx1, tpx2 and both) and with a gene involved in the ascorbic acid
synthesis (GalUR) through successive transformation, to use them
for future biotechnological applications. Stable transgenic HR
clones of tobacco which expressed the foreign genes tpx1, tpx2in vitro growth without hormones and
biochemical and genetic stability. Genetic engineering has allowed
to transform plants with foreign genes to give or to improve
special metabolic features. The establishment of transgenic HR
can be obtained through a unique step of transformation, using
genetically modified A.rhizogenes, or by an unusual method with
two steps using A. tumefaciens and A. rhizogenes. The latter
technique is called successive transformation. The aim of this
work was to obtain transgenic HR cultures with peroxidases genes
(tpx1, tpx2 and both) and with a gene involved in the ascorbic acid
synthesis (GalUR) through successive transformation, to use them
for future biotechnological applications. Stable transgenic HR
clones of tobacco which expressed the foreign genes tpx1, tpx2A.rhizogenes, or by an unusual method with
two steps using A. tumefaciens and A. rhizogenes. The latter
technique is called successive transformation. The aim of this
work was to obtain transgenic HR cultures with peroxidases genes
(tpx1, tpx2 and both) and with a gene involved in the ascorbic acid
synthesis (GalUR) through successive transformation, to use them
for future biotechnological applications. Stable transgenic HR
clones of tobacco which expressed the foreign genes tpx1, tpx2A. tumefaciens and A. rhizogenes. The latter
technique is called successive transformation. The aim of this
work was to obtain transgenic HR cultures with peroxidases genes
(tpx1, tpx2 and both) and with a gene involved in the ascorbic acid
synthesis (GalUR) through successive transformation, to use them
for future biotechnological applications. Stable transgenic HR
clones of tobacco which expressed the foreign genes tpx1, tpx2tpx1, tpx2 and both) and with a gene involved in the ascorbic acid
synthesis (GalUR) through successive transformation, to use them
for future biotechnological applications. Stable transgenic HR
clones of tobacco which expressed the foreign genes tpx1, tpx2GalUR) through successive transformation, to use them
for future biotechnological applications. Stable transgenic HR
clones of tobacco which expressed the foreign genes tpx1, tpx2tpx1, tpx2
and both genes and tomato HR which expressed GalUR gene were
established. As it could be analysed by PCR, the clones contained
not only the rol C gene, which is characteristic of HR but also the
foreign genes integrated in the transgenic plants used in the first
step. Growth index, enzyme activities and the isoelectric focusing
zymograms of the enzymes were analysed for transgenic and wild
type clones. The results showed the efficiency of the successive
transformation technique to obtain stable transgenic HR clones.
This method constitutes a useful tool to give or to improve HR
cultures features for biotechnological applications.GalUR gene were
established. As it could be analysed by PCR, the clones contained
not only the rol C gene, which is characteristic of HR but also the
foreign genes integrated in the transgenic plants used in the first
step. Growth index, enzyme activities and the isoelectric focusing
zymograms of the enzymes were analysed for transgenic and wild
type clones. The results showed the efficiency of the successive
transformation technique to obtain stable transgenic HR clones.
This method constitutes a useful tool to give or to improve HR
cultures features for biotechnological applications.rol C gene, which is characteristic of HR but also the
foreign genes integrated in the transgenic plants used in the first
step. Growth index, enzyme activities and the isoelectric focusing
zymograms of the enzymes were analysed for transgenic and wild
type clones. The results showed the efficiency of the successive
transformation technique to obtain stable transgenic HR clones.
This method constitutes a useful tool to give or to improve HR
cultures features for biotechnological applications.