Orally-Induced Intestinal CD4+ CD25+ FoxP3+ Treg Controlled Undesired Responses towards Oral Antigens and Effectively Dampened Food Allergic Reactions

SMALDINI P; ORSINI DELGADO L; FOSSATI, CA; DOCENA GH,

PLOS ONE

PUBLIC LIBRARY SCIENCE

Lugar: San Francisco; Año: 2015 vol. 10 p. 1 - 14

1932-6203

Theinduction of peripheral tolerance may constitute a disease-modifying treatmentfor allergicpatients.We studied how oral immunotherapy (OIT) with milk proteins controlled allergyinsensitized mice (cholera toxin plus milk proteins) upon exposure to theallergen. Symptomswerealleviated, skin test was negativized, serum specific IgE and IgG1 wereabrogated,asubstantial reduction in the secretion of IL-5 and IL-13 by antigen-stimulatedspleencells was observed, while IL-13 gene expression in jejunum was down-regulated,andIL-10 and TGF-β were increased.In addition, we observed an induction of CD4+CD25+-FoxP3+cells and IL-10- and TGF-β-producingregulatory T cells in the lamina propria.Finally,transfer experiments confirmed the central role of these cells in toleranceinduction.Wedemonstrated that the oral administration of milk proteins pre- orpost-sensitization controlledtheTh2-immune response through the elicitation of mucosal IL-10- and TGF-β-producingTregs that inhibited hypersensitivitysymptoms and the allergic response.