Characterization of a 18 Kda Brucella Cytoplasmic Protein Wich Appears To Be a Serological Marker Of Active Infection of Both Human and Bovine Brucellosis

F G, GOLDBAUM; LEONI J,; WALLACH JC,; CARLOS ALBERTO FOSSATI

JOURNAL OF CLINICAL MICROBIOLOGY

AMER SOC MICROBIOLOGY

Año: 1993 vol. 31 p. 2141 - 2145

0095-1137

Some anticytoplasmic protein monoclonal antibodies (MAbs) from mice immunized by infection with BruceUla ovis cells have been obtained. One of these MAbs, B124, was used to purify by immunoaffinity a protein with a pI of 5.6 and a molecular mass of 18 kDa. This protein was present in all of the rough and smooth BruceUla species studied, but it could not be detected in Yersinia enterocolitica 09. Three internal peptides of this protein were partially sequenced; no homology with other bacterial proteins was found. The immunogenicity of the 18-kDa protein was studied with both human and bovine sera by a capture enzyme-linked immunosorbent assay system with MAb B124