INVESTIGADORES
GAGLIARDINO Juan Jose
artículos
Título:
Islet NADPH oxidase activity is modulated unevenly by different secretagogues
Autor/es:
REBOLLEDO OR ; RASCHIA MA; BORELLI MI; GARCÍA ME; GAGLIARDINO JJ
Revista:
ENDOCRINE
Editorial:
HUMANA PRESS INC
Referencias:
Año: 2010 vol. 38 p. 309 - 311
ISSN:
0969-711X
Resumen:
NADPH oxidase expression and activity has been measured in pancreatic islets under normal conditions, but its potential modulatory role upon insulin secretion remains unclear. We have currently studied NADPH oxidase activity in islets isolated from normal rats as well as the effect of its inhibition upon insulin secretion stimulated by different secretagogues. Glucose, arginine, fatty acids and KCl increased islet NADPH oxidase activity in a stimulus-dependent manner. DPI inhibited in different proportion such increase and affected unevenly insulin secretion: it decreased the effect of high glucose, increased that of oleic acid and KCl without changing that induced by palmitic acid. Our data provide evidence that the contribution of NADPH activity to ROS production in normal rat islets as well as its effect upon insulin secretion, is uneven and highly stimulus-dependent.
