Risedronate functionalized layered double hydroxides nanoparticles with bone targeting capabilities

DARIANA ARISTIZABAL BEDOYA; CECILIA VASTI; RICARDO ROJAS; CARLA E. GIACOMELLI

APPLIED CLAY SCIENCE

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2017 vol. 141 p. 257 - 264

0169-1317

abstractLayered double hydroxidesnanoparticles (LDH-NPs) are increasingly studied as drug nanocarriers for cellular de- livery. Nevertheless, stable functionalizations providing targeting capabilities without disrupting the size of the carriers are necessary to achieve optimized performance. Here, LDH-NPs were functionalized with risedronate (Ris) to improve the osteotropicity of the nanocarrierswithout altering the nanosized distribution. Ris is a nitro- gen containing bisphosphonate with rich acid-base reactivity that can lead Ris functionalized LDH-NPs also as pH-responsive drug nanocarriers. The current work is focused on the strategy to synthesize functionalized LDH-NPs with a maximum adsorption and a minimum intercalation of Ris while maintaining their nanosize. The speciation and interactions of Ris at the surface of LDH-NPswere analyzed using Raman microscopywhereas the functionalization stability and size distributionwere checked in simulated biological media. Finally, pH sen- sitivity and hydroxyapatite binding capacity of Ris functionalized LDH-NPswere evaluated. HRis3− anionswere incorporated to the LDH-NPs surfacewith high affinity providingwith a negative zeta potential that controlled the size at around 100 nm. The size of Ris functionalized LDH-NPs was not affected by the high ionic strength or the presence of proteins in simulated biological media. Further, the functionalization was stable against pro- tein adsorption and anionic exchange. As expected, Ris functionalized LDH-NPs are bioresponsive with a high sensitivity for pH changes and specificaffinity for hydroxyapatite, which makes them appealing drug nanocarriers for newbone therapies.