Modeling and estimation of production rate for the production phase of non-growth associated high cell density processes

MARTIN JAMILIS; FABRICIO GARELLI; MD. SALATUL ISLAM MOZUMDER; TERESITA CASTAÑEDA; HERNÁN DE BATTISTA

BIOPROCESS AND BIOSYSTEMS ENGINEERING

SPRINGER

Lugar: Berlin; Año: 2015 vol. 38 p. 1903 - 1918

1615-7591

This paper addresses the estimation of the specificproduction rate of intracellular products and the modelingof the bioreactor volume dynamics in high cell density fedbatch reactors. In particular, a new model for the bioreactorvolume is proposed, suitable to be used in high cell density cultures where large amounts of intracellular productsare stored. Based on the proposed volume model, two formsof a high order sliding mode observer are proposed. Eachform corresponds to the cases with residual biomass concentration or volume measurement respectively. The observersachieve finite time convergence and robustness to processuncertainties as the kinetic model is not required. Stabilityproofs for the proposed observer are given. The observer algorithm is assessed numerically and experimentally.