Alteraciones hemodinámicas y tubulares renales posteriores a obstrucción ureteral aguda en ratas

VILLAR, SILVINA RAQUEL; BRANDONI ANABEL; QUAGLIA, NORA; TORRES, ADRIANA MONICA

Rosario, Santa Fe.

Congreso; V Congreso XXII Reunión Anual de la Sociedad de Biología de Rosario.; 2002

Sociedad de Biología.(SBR)

Bilateral Ureteral Obstruction (OUB) may cause acute renal insuficiency. The physiophatologic mechanisms involved in short time OUB have not been completely elucidated. The aim of this study was to evaluate some parameters of renal function after acute OUB. Adult male Wistar rats were employed. Both ureters were ligated during 24 h, then were released and the studies were done after 24 h (n = 5). A parallel group of Sham rats (Sh, n = 6) was employed. Plasma urea levels were determined before the obstruction (t= 0 h), the release (t = 24 h) and the experiments ( t = 48) by spectrophotometric techniques (Ur, g/l). P-aminohippurate renal clearance (Cl PAH, ml/min/100 g), glomerular filtration rate (GFR, ml/min/100 g), fractional excretion of water (FE% H2O), sodium (FE% Na), potassium (FE% K) and the ratio urine to plasma osmolality (U/P) were determined employing conventional clearance techniques. Data were analysed with unpaired "t" Student Test or ANOVA, and p < 0.05 (*) were considered statistically significative.Results: Ur Sh= (t=0 h): 0.54 ± 0.04, (t=24 h): 0.51 ± 0.03, (t=48 h): 0.72 ± 0.06, Ur OUB= (t=0 h): 0.56 ± 0.03, (t=24 h): 3.32 ± 0.14 *, (t=48 h): 2.91 ± 0.30*(*p<0.05 vs t=0); Cl PAH: Sh = 5.26 ± 0.78; OUB = 0.49 ± 0.18*;GFR: Sh = 0.582 ± 0.053, OUB = 0.127 ± 0.020*; FE H2O%: Sh = 0.876 ± 0.089, OUB = 9.86 ±1.57*; FE%Na: Sh = 1.28 ± 0.19, OUB = 50 ± 11*; FE%K: Sh = 32 ± 4; OUB = 88 ± 5*; U/P: Sh = 3.71 ± 0.44; OUB = 1.56 ±0.06*. Marked renal hemodynamic alterations and modifications in the renal handling of water and electrolytes are observed in this experimental model of short time OUB, which persists after release of obstruction.