ANDROGENIC REGULATION OF ACYL-COA SYN- THETASE 4 IN BREAST CANCER CELLS

MELINA A DATTILO; PAULA LOPEZ; EVELYN ADLER; YANINA BENZO; MARIA MERCEDES BIGI; ESTEFANIA CLAVERO; CRISTINA PAZ.; ERNESTO J. PODESTÁ; PAULA M MALOBERTI

Congreso; LXVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2022

It is known that ACSL4 overexpression is involved in the develop-ment of a very aggressive phenotype in breast and prostate cancer.Androgen receptor (AR) expression or androgenic stimulation de-crease mRNA levels of ACSL4 in prostate cancer cells. However,the effects of androgenic regulation in breast cancer cells have notyet been described. The aim of this work is to study whether AR andandrogens regulate ACSL4 transcription in breast cancer cells. Weobserved that AR mRNA is detected in the slightly aggressive breastcancer cell line MCF-7. In contrast, this expression is not detectablein the very aggressive cell line MDA-MB-231. Bioinformatic analysisperformed with the Genomatix tool and contrasted with the AlggenPromo software, showed one AR element (ARE) in a 1.8 kb frag-ment of the human ACSL4 promoter with a score close to 1. Tran-sient transfection of AR in MDA-MB-231 cell line reduced ACSL41,8 kb promoter activity (p