INVESTIGADORES
BERLI Claudio Luis Alberto
artículos
Título:
Laser fabrication of micropores and their integration to microfluidic platforms for DNA electrophoresis
Autor/es:
B. LERNER; M. S. PEREZ; P. A. KLER; C. L. A. BERLI; A. F. ORDOÑEZ ARIAS; F. SACCO; C. TORO; C. A. RINALDI
Revista:
MICROSYSTEM TECHNOLOGIES-MICRO-AND NANOSYSTEMS-INFORMATION STORAGE AND PROCESSING SYSTEMS
Editorial:
SPRINGER
Referencias:
Año: 2012 vol. 18 p. 429 - 435
ISSN:
0946-7076
Resumen:
The work presents an alternative method for the manufacture of micropores by using
the combination of laser ablation and wet etching. The process of laser ablation was
done on silicon (Si) wafers with silicon nitride (Si3N4) used as a sacrificial layer. The
size of the pores was carefully controlled by following an optical technique. The
method exhibits a series of advantages in relation micromachining techniques
previously used. The fabricated pores were then integrated to polydimethylsiloxane
(PDMS) microchannels, which constitutes a novel setup to be considered for
microfluidic applications. Furthermore, as the system is addressed to study the
electrically-driven transport of macromolecules, deoxyribonucleic acid (DNA)
electrophoresis was performed in the fabricated microsystems to prove the principle.
Also for these purposes, the electric field throughout the microchannel and pore region
was studied in details by using numerical simulations.