Sugarcane Systemic Diseases Diagnosis At Estación Experimental Agroindustrial Obispo Colombres (EEAOC).

FILIPPONE, M. P., NOGUERA, A., SALGADO, M., VIRUEL, E., PERERA, M. F., RAMALLO, J. Y CASTAGNARO, A.

BIOCELL

Inca Editorial

Lugar: Buenos Aires; Año: 2006 vol. 30 p. 212 - 212

0327-9545

Sugar industry is one of the most important in Tucumán. Therefore, to know the crop health situation is fundamental to prevent or reduce the losses caused by diseases. Consequently, a precise and highly sensitive diagnosis method is required. “Enzime Linked Inmunosorbent Assay” (ELISA) inmunoenzimatic technique and its variants, are the most commonly used, although Polymerase Chain Reaction (PCR) molecular technique has been found to improve pathogen detection. At EEAOC, ELISA and since 2005 PCR, are used for optimizing disease diagnosis. Thus, the molecular diagnosis of four diseases caused by Leifsonia xyli sp. xyli, Xanthomonas albilineans, Sugar cane Mosaic Virus (ScMV), Sugar cane Yellow Leaf Virus (ScYLV), was optimized. The analysis of 160 samples with both diagnosis techniques showed no differences in bacterial pathogen detection, whereas with PCR a greater number of positive samples for ScMV was determined. From this results, we suggest to apply the serological technique and then PCR in the negative samples; it will allow a balance between sensitivity and analysis cost.