ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at the efferent-inner hair cell synapse in the developing inner ear

ALVAREZ HEDUAN F; GOUTMAN J; ELGOYHEN AB

Congreso; XXIX Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; 2014

Before the onset of hearing (postnatal day (P) 12 in mice) inner hair cells (IHCs) are transiently innervated by medial olivocochlear (MOC) efferent fibers. Acetylcholine (ACh) released by these fibers activates 910 nicotinic receptors coupled to SK2 calcium-activated potassium channels, leading to inhibitory post synaptic currents (IPSCs). During this period, IHCs fire spontaneous sensory-independent action potentials that are required for normal development of the auditory pathway. This activity is driven and/or modulated by ATP released from cochlear supporting cells. ACh release from efferent fibers also contributes to this modulation. By electrically stimulating MOC fibers and recording IPSCs from IHCs, we previously showed that ATP decreases ACh release in a reversible and concentration-dependent manner. In this work, we demonstrate that this effect is through P2Y receptor activation, as the specific P2Y agonist 2-MeSADP mimicked the effect of ATP. Moreover, the non-hydrolyzable ATP analog ATPS decreased ACh release as well, indicating that this modulation is driven by ATP itself. We further tested if adenosine can also modulate ACh release. Adenosine reversibly decreased the quantal content. CGS15943, a specific P1 receptor antagonist, abolished the effect of adenosine. On the other hand NECA, a specific P1 agonist, decreased ACh release. Our results suggest that both ATP and adenosine inhibit ACh release at the MOC-IHC synapse through the activation of pre-synaptic P2Y and P1 receptors, respectively.