HEK-blue TLR4 reporter cell line potential to distinguish functional changes in stool?s microbiota

GIRARDI JI; ERREA AJ; ROLNY IVANNA; RUMBO MARTIN

Tucumán

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Inmunología; 2019

Sociedad Argentina de Inmunología

Ambiental factors may condition the composition of intestinal microbiota with important impact in a wide variety of diseases, including type 2 diabetes, obesity, cancer, Crohn's disease and even neurodegenerative diseases. Different antibiotic treatments may have differential impact on microbiota functional properties.The aim of our work is to characterize the potential of a HEK-Blue hTLR4 reporter cell line to assert changes in intestinal microbiota from stool in an antibiotic-induced dysbiosis model in mice. We treated mice with ampicillin, vancomycin or azithromycin and compared stool's TLR-4 agonist activity and microbiota composition assessing stool's DNA by qPCR. Non-treated mice were used as controls.We observed a differential stimulatory activity of hTLR-4 reporter cell line depending on the antibiotic treatment. While stools from azithromycin-treated mice showed lower TLR-4 agonist activity than controls, vancomycin and ampicillin promoted an increase in stimulatory potential (p< 0.0001). Microbiota analysis showed no changes in Firmicutes, a decrease on Bacteroidetes and a decreasing tendency of Proteobacteria population for azithromycin. Vancomycin and ampicillin, although both increased hTLR-4 agonist activity in stool, showed different changes in microbiota. Both vancomycin and ampicillin induced a relative decrease in Bacteroidetes (p