Molecular characterization of Fusarium spp. causing peanut brown root rot and strategies for their biocontrol

GANUZA, MAURICIO; PASTOR, NICOLÁS; CASASNOVAS, FRANCISCO; REYNOSO, MARÍA MARTA; ROVERA, MARISA; TORRES, ADRIANA

Berlin

Congreso; XVIII International Plant Protection Congress (IPPC) 2015; 2015

International Association for the Plant Protection Sciences (IAPPS)

Argentina is a major peanut-producing country. During the 2011/12 season, peanut production exceeded 680 000 tons. Most of the peanuts are exported to the European Union and the USA. Diseases caused by soil pathogenic fungi limit peanut production.Peanut brown root rot (PBRR) was first described in Argentina in 1992. The etiological agent responsible was reported as Fusarium solani (Mart.) Appel and Wollenweber, but the pathogen was not well described. Koch?s postulates were completed with strains from this group to show that these strains are responsible for this disease. We used a combination of morphological and molecular markers, amplified fragment length polymorphism (AFLPs), and molecular sequence analysis of internal transcribed spacer region (ITS) of the translation elongation factor 1-alpha gene (TEF-1α), and the α-tubulin gene in 15 Fusariumisolates obtained from peanut plants exhibiting symptoms of root rot, to characterize strains of this pathogen in the Fusarium solani species complex (FSSC). Also, evaluation of the efficacy of bioagents application against PBRR disease incidence was carried out under greenhouse conditions.