Photodynamic inactivation of Candida albicans sensitized by porphyrins

ÁLVAREZ M G; FUNES M; OTERO L A; DURANTINI E N

Mendoza Argentina

Congreso; XXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2008

Sociedad de Biología de Cuyo

<!-- /* Font Definitions */ @font-face {font-family:"Arial Unicode MS"; panose-1:2 11 6 4 2 2 2 2 2 4; mso-font-charset:128; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1 -369098753 63 0 4129279 0;} @font-face {font-family:"@Arial Unicode MS"; panose-1:2 11 6 4 2 2 2 2 2 4; mso-font-charset:128; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1 -369098753 63 0 4129279 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB; mso-fareast-language:ES;} @page Section1 {size:21.0cm 842.0pt; margin:109.15pt 106.3pt 109.15pt 106.3pt; mso-header-margin:36.0pt; mso-footer-margin:51.05pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Photodynamic inactivation of Candida albicans sensitized by porphyrins Álvarez M G, Funes M, Otero L A, Durantini E N Dpto de Química, Universidad Nacional de Río Cuarto, Agencia Postal Nro. 3, X5804BYA Río Cuarto, Córdoba, Argentina. E-mail: malvarez@exa.unrc.edu.ar Antimicrobial resistance is a growing problem that complicates the treatment of nosocomial and community-acquired infections. In the last years, resistance of C. albicans is increasing against traditional antifungal, such as fluconazole. The search for new therapeutic approaches is stimulated by the fact that standard antifungal treatments are prolonged and expensive and the appearance of drug resistant strains is more frequent in patients. Photodynamic inactivation (PDI) represents an interesting alternative that involves the pre-treatment with a sensitizer, which it accumulates in microbial cells. The exposure to visible light leads to the generation of singlet oxygen (1O2), which produces photodamage and destruction of cells. In this work was studied the PDI of C. albicans sensitized by a tetracationic porphyrin (P) and its metal complex with Pd(II). The cultures were treated with different concentration of sensitizer (1-10 mM) for 30 min at