MODULATORY EFFECT OF TESTOSTERONE ON THE THERMOGENIC ACTIVITY OF BEIGE ADIPOCYTES FROM RETROPERITONEAL ADIPOSE TISSUE

HARNICHAR ALEJANDRO EZEQUIEL; ZUBIRÍA, MARÍA GUILLERMINA; MARIA AMANDA REY; SPINEDI, EDUARDO; GIOVAMBATTISTA, ANDRÉS

Mar del Plata

Congreso; LXI Reunion Científica Anual de la Soc. Arg. de Investigaciones Clínica; 2016

Itis well known that androgens modulate adipose tissue (AT) distribution andfunction. We earlier showed the effect of the lack of testosterone (T) onretroperitoneal AT (RPAT) function from orchidectomized rats and the inhibitoryaction of T on the adipogenic potential of adipocyte precursor cells (APCs) in vitro. We now studied the effect ofT, in vivo and in vitro, on the thermogenic activity of beige adipocytes fromRPAT. For this aim, RPAT pads were dissected from adult male control (CTR),pre-pubertally orchidectomized (ODX) and pair-fed control (CTR-PF) rats. Padswere processed for histology analysis and UCP-1 quantification (RT-PCR). Inaddition, APCs from adult male rats were isolated and cultured up toconfluence, then cells were induced to differentiate (4 days) with apro-browning cocktail in the absence or presence of 0.1 µM T (basal (B) or T,respectively); thereafter cells remained in culture medium without or with T.On differentiation day 8, we added 10 µM forskolin (FSK) for 4 hs to a subsetof B or T cells: B without FSK (B-B), B with FSK (B-FSK), T without FSK (T-B),T with FSK (T-FSK). Cells were then processed to quantify UCP-1. We found thatT decreased UCP-1 expression in in vitrodifferentiated adipocytes (p