Spexin Improves Metabolic Deleterious Efects Caused By Fructose Rich Diet Intake

ZUBIRÍA, MARÍA GUILLERMINA; GAMBARO, SABRINA ELIANA; GIORDANO ALEJANDRA PAULA; MARIA AMANDA REY; GIOVAMBATTISTA, ANDRÉS

Congreso; Reunión Anual de Sociedades de Biociencias; 2021

Spexin (SPX) is a novel hormone distributed in numerous tissues, including white adipose tissue (AT). SPX regulates lipid and carbohydrate metabolism, caloric intake and body weight loss. Here, we evaluate the ability of SPX to improve metabolic profile and visceral AT function in fructose rich diet (FRD) obese mice. Four male mice (C57BL/6J) groups were studied: CTR mice, FRD mice (10 weeks of 20% w/v fructose in drinking water), and two similar groups that were treated or not with SPX for ten days prior to the end of the protocol (ip. 29 µg/kg/day; CTR-SPX and FRD-SPX). Body weight and caloric intake were recorded every day. Glucose Tolerance Test (GTT) was performed. Plasma was collected for triglycerides (TG) and glucose (GLU) levels quantification. AT depots (Inguinal (IAT), retroperitoneal (RPAT) and Epididymal (EAT)) were dissected, weighted and EAT was used for quantification of Ob, Adiponectin, PPARγ2 and GALR2 by qPCR. Two-way ANOVA was used to determine variable (SPX and FRD) and interaction (FRDxSPX) effects. SPX caused weight loss (p