Changes in phosphomannosyl ligands correlate with cation-dependent mannose-6-phosphate receptors in rat liver during perinatal development

ROMANO, P.; JOFRÉ G., CARVELLI L, LÓPEZ, C; SARTOR T; AND SOSA MA

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Elsevier

Lugar: Philadelphia ; Año: 2006 vol. 344 p. 605 - 611

0006-291X

The co-existence of two mannose-6-phosphate receptors (CD-MPR and CI-MPR) in most cell types is still a dilemma to be resolved. 13 In this study, some parameters were measured to explore lysosomal apparatus evolution in rat liver during perinatal development, andIn this study, some parameters were measured to explore lysosomal apparatus evolution in rat liver during perinatal development, and 14 establish a possible involvement of CD- and/or CI-MPR in lysosome maturation. Activity of four acid hydrolases was measured in theestablish a possible involvement of CD- and/or CI-MPR in lysosome maturation. Activity of four acid hydrolases was measured in the 15 whole organ at different ages and it was found that N-acetyl-b-D-glucosaminidase (NAG), b-galactosidase, and b-glucuronidase changewhole organ at different ages and it was found that N-acetyl-b-D-glucosaminidase (NAG), b-galactosidase, and b-glucuronidase change 16 during development, reaching a peak at the 10th day after birth. These results correlated with the expression and binding properties ofduring development, reaching a peak at the 10th day after birth. These results correlated with the expression and binding properties of 17 CD-MPR previously reported. We also used a method that recognizes phosphomannosylated ligands by using purified biotinylated CI-CD-MPR previously reported. We also used a method that recognizes phosphomannosylated ligands by using purified biotinylated CI- 18 MPR as a probe, and found that the highest concentrations of ligands also appear around the 10th day. Binding assays were also carriedMPR as a probe, and found that the highest concentrations of ligands also appear around the 10th day. Binding assays were also carried 19 out, incubating endogenous NAG from 10-day-old and adult rats with membranes from their respective ages, and the results indicatedout, incubating endogenous NAG from 10-day-old and adult rats with membranes from their respective ages, and the results indicated 20 that cation-dependent mannose-6-phosphate receptor (CD-MPR) has more impact on trafficking of the enzyme at the 10th day afterthat cation-dependent mannose-6-phosphate receptor (CD-MPR) has more impact on trafficking of the enzyme at the 10th day after 21 birth. We concluded that lysosome maturation in the rat liver occurs around the 10th day after birth, and that the CD-MPR may par-birth. We concluded that lysosome maturation in the rat liver occurs around the 10th day after birth, and that the CD-MPR may par- 22 ticipate in that event.ticipate in that event. 23 2006 Elsevier Inc. All rights reserved.2006 Elsevier Inc. All rights reserved.