INVESTIGADORES
ROMANO Patricia Silvia
artículos
Título:
Changes in distribution of phosphomannosyl receptors during maduration of rat spermatozoa
Autor/es:
BELMONTE SA; ROMANO PS; FORNÉS MW; SOSA MA
Revista:
BIOLOGY OF REPRODUCTION
Editorial:
SOC STUDY REPRODUCTION
Referencias:
Lugar: Madison; Año: 2000 vol. 63 p. 1172 - 1178
ISSN:
0006-3363
Resumen:
The aim of the present work was to study the distribution of
the cation-independent (CI) and cation-dependent (CD) mannose-
6-phosphate receptors (MPRs) in spermatozoa obtained
from either rete testis or three regions of rat epididymis. We
observed that both receptors underwent changes in distribution
as spermatozoa passed from rete testis to cauda epididymis. CIMPR
was concentrated in the dorsal region of the head in rete
testis sperm and that this labeling extended to the equatorial
segment of epididymal spermatozoa. CD-MPR, however,
changed from a dorsal distribution in rete testis, caput, and corpus
to a double labeling on the dorsal and ventral regions in
cauda spermatozoa. The percentages of spermatozoa that
showed staining for either CI-MPR or CD-MPR increased from
rete testis to epididymis. The observed changes were probably
the result of a redistribution during transit rather than an unmasking
of receptors. The fluorescence corresponding to CDMPR
and CI-MPR on the dorsal region disappeared when caudal
spermatozoa underwent the acrosomal reaction. Receptors were
localized on the plasmalemma of spermatozoa, as observed by
immunoelectron microscopy. Changes in distribution may be related
to a maturation process, which suggests new roles for the
phosphomannosyl receptors.