REGENERATION PROTOCOLS FOR TRANSFORMED ROOTS OF PETUNIA AXILLARIS (LAM.) BRITTON, STERN & POGGENB

MARIANA PÉREZ DE LA TORRE; ANDREA COVIELLA, VERÓNICA BELLA, SILVINA SOTO; SANDRA IRENE PITTA-ALVAREZ

Mar del Plata

Encuentro; VIII Encuentro Latinoamericano y del Caribe de Biotecnología REDBIO-Argentina 2013; 2013

REDBIO

The development of national varieties of ornamental plants with compact architecture is relevant for local producers because they have more acceptance and commercial value. Since plants regenerated from hairy roots exhibit dwarfism, one genotype of Petunia axillaris was transformed with Agrobacterium rhizogenes LBA15834. Transformation was confirmed by PCR reaction with Rol A, Rol B, Rol C, Rol D and Vir C primers. For regeneration, naphtalen-acetic acid (NAA) and 6-bencil aminopurine (BAP) were used in the following combinations: MS1: Control, MS2: 0.5 mg/l BAP, MS3: 2 mg/l BAP, MS4: 0.5 mg/l NAA, MS5: 2 mg/l NAA, MS6: 0.5 mg/L NAA + 0.5 mg/L BAP, MS7: 0.5 mg/l NAA + 2 mg/l BAP, MS8: 2 mg/l NAA + 0.5 mg/l BAP and MS9: 2 mg/L NAA + 2 mg/l BAP. Preliminary results showed the MS4, MS6 and MS8 were the most promising combinations. For plantlet regeneration, the calli obtained were transferred to MS hormone-free medium containing 30 g/l sucrose and 7.5 g/l agar. Calli from treatment MS8 presented root tips and adventitious root-like structures. At the moment, the last regeneration step is underway.