ICMT ROLE IN THE RESPONSE TO RENAL ISCHEMIC DAMAGE

BORINI-ETICHETTI, CARLA; FUSSI, MARÍA FERNANDA; MONASTEROLO, LILIANA ALICIA; LAROCCA, M. CECILIA; MOLINAS, SARA MARÍA; GIRARDINI, JAVIER

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

Renal ischemia-reperfusion (IR) is one of the main causes of acute kidney injury (AKI). Rho GTPases, RhoA and Cdc42, act as regulators of various processes that directly impact the ability of the renal epithelium to regenerate. A key regulator of these Rho GTPases is the enzyme Isoprenyl-cysteine carboxy methyltransferase (ICMT), which participates in the post-translational modification of their C-terminus. The aim of this work was to investigate the role of ICMT in the epithelial tubular cell response to ischemic AKI. We previously demonstrated that, in IR rats, the early stage of renal damage is associated with a decrease in RhoA, Cdc42 and ICMT and the stage of marked regeneration is associated with an increment of these proteins. We also demonstrated that Angiotensin II type 2 receptor activation by its agonist, C21, prevented renal IR tubular epithelial cell damage in rats. Here, we found that C21 (1 mg/kg/day, i.p.) prevented the decrease in ICMT abundance in Wistar rats submitted to 40 min unilateral renal ischemia + 1 day of reperfusion (-55%, p