RENAL EXPRESSION OF RhoGTPases AND ITS MODULATOR, ICMT, IN THE EVOLUTION OF TUBULAR EPITHELIAL RESPONSE TO ACUTE RENAL DAMAGE

BUONO, GABRIEL M; FUSSI, MARÍA FERNANDA; MARQUEZ, SUSANA B; RIBAVELLA MAKNIS, TOMAS; GIRARDINI, JAVIER E.; LAROCCA, M. CECILIA; MONASTEROLO, LILIANA ALICIA; MOLINAS, SARA MARÍA

Congreso; Reunión anual de la Sociedad Argentina de Fisiología; 2019

Acute kidney injury (AKI) is still a significant medical problem. Renal ischemia-reperfusion (IR) injury is one of the main causes of AKI. RhoA and Cdc42 are RhoGTPases involved in the maintenance of epithelial integrity in renal tubules. Our aim was to study the signaling pathways involved in the development of AKI by characterizing RhoGTPase pathway in the IR model. Male Wistar rats (n=6 per group) were subjected to 40 min of unilateral renal ischemia followed by 1 (I1) or 7 (I7) days of reperfusion. Controls underwent sham operation. Histological studies showed extensive tubular damage in I1 kidneys associated with decreased glomerular filtration rate (GFR). In agreement with studies performed at shorter reperfusion times, we found that cortical expression of RhoA (-65% p