POLYLACTIC ACID SHEETS SEEDED WITH GENETICALLY MODIFIED OVINE DIAPHRAGMATIC MYOBLASTS FOR MYOCARDIAL REGENERATION. PRELIMINARY RESULTS.

CARLOS SEBASTIÁN GIMENEZ; FERNANDA DANIELA OLEA; PAOLA LOCATELLI; MARIA DEL ROSARIO BAUZA; RICARDO A. DEWEY; FLORENCIA MONTINI BALLARIN; GUSTAVO ABRAHAM; ALEJANDRO ORLOWSKI; ANDREA DE LORENZI; LUIS CUNIBERTI; ALBERTO CROTTOGINI

Congreso; LXI Reunión Cientifica Anual Sociedad Argentina de Investigación Clínica (SAIC).; 2016

SAIC

POLYLACTIC ACID SHEETS SEEDED WITH GENETICALLY MODIFIED OVINE DIAPHRAGMATIC MYOBLASTS FOR MYOCARDIAL REGENERATION. PRELIMINARY RESULTS.We tested the hypothesis that polylactic acid (PLA) sheets seeded with diaphragmatic myoblasts (DM) overexpressing connexin-43 (Cx43) would decrease infarct size and improve left ventricular (LV) function in sheep with chronic myocardial infarction (MI). Forty minutes after coronary artery ligation, PLA sheets without DM (PLA, control group, n=4) or PLA sheets with DM overexpressing Cx43 (PLA-DM-Cx43 group, n=4) were sutured covering the infarcted zone and 1 cm of the adjacent peri-infarct zone. Baseline echocardiograms confirmed that all animals displayed normal LV function prior to infarction. Cardiac magnetic resonance with gadolinium enhancement was performed at 3 and 45 days after MI to determine infarct size and LV function early and late after treatment, respectively. Blinded data analysis revealed that infarct size expressed as ml of infarcted tissue decreased in the PLA-DM-Cx43 group from 8.0±1.83 ml at day 3 to 6.25 ±2.2 ml at day 45 (p