“Expression of progesterone membrane receptor gene in pig’s oviduct

MARTÍN E. ARGAÑARAZ; MARIELA ROLDÁN OLARTE; DORA C. MICELI

Tafi del Valle, Tucuman, Argentina

Jornada; XVIII Jornadas Científicas de la Asociación de Biología de Tucumán; 2001

Asociación de Biología de Tucumán

The steroid hormones act modulating the nuclear transcription and the synthesis of proteins through nuclear receptors. But also they act through specific membrane receptors that trigger fast effects. It is known that in pig´s oviduct the steroid hormones provide an apt milieu for the transport of gametes, the qualification of the spermatozoa, the fertilization and the embryogenesis early stages. Since great part of these processes are regulated by the progesterone, is important to investigate the molecular mechanisms involved in this regulation. The objective of this work was to detennine the genetic expression of the progesterone membrane receptor in pig´s oviduct. The oviducts were classified in different stages according to the estrous cycle. After extraction and purification of the RNA, RT-PCR assays showed an specific amplified band. The results indicated that the gene of the progesterone receptor is expressed in all the stages of the sexual cycle. In comparison with the expression of b-actin taking as a control of constitutive expression, semi-quantitative differences were not early observed. Since the actin is also involved in phenomena of cellular response, we thought that it is necessary to use another constitutive gene to study the progesterone membrane receptors regulation during the sexual cycle in further assays for conflrming the differential expression of this gene.