“Involvement of NAD-Poly (ADP-Ribose) metabolism on p53 regulation by topotecan

E. I. YSLAS P. A. VALDECANTOS, M. ROLDÁN OLARTE AND C. MERCHED WHITACRE.

Orlando, Florida, EEUU

Congreso; Florida Academy of Sciences 67th Meeting; 2003

University of Central Florida

Involvement of NAD-Poly(ADP-Ribose) metabolism on p53 regulation by topotecan. E.I YSLAS (1),  P.A. VALDECANTOS (2), M. ROLDAN-OLARTE (2), AND C. MERCHED WHITACRE (3). (1) Mol. Biol. Dept., University of Rio Cuarto, Argentina (2) Dev. Biol. Dept., National University of Tucuman, Argentina (3) SNHS-Biology, Barry University 11300 NE 2nd Avenue, Miami Shores, FL 33161.  We have evaluated the effect of NAD/poly(ADP-ribose) polymerase (PARP) metabolism deficiency on Topotecan induced apoptosis in HeLa cervical cancer cells. Cells grown in the absence or presence of NAD were treated during 24-48 hs with increasing doses of topotecan (0.1-1 μM).  Our studies suggest that NAD/PARP synthesis may be involved in TPT-induced apoptotic pathway and p53 regulation (Supported by NIH- NCI-K1CA77065).