INVESTIGADORES
ROLDAN OLARTE Eugenia Mariela
artículos
Título:
Raman Microscopy Analysis of the Biochemical Changes in the Cytoplasm of Bovine Oocytes Induced by an In Vitro Maturation Process: Interference of the Zona Pellucida
Autor/es:
JIMENEZ, LUIS EMANUEL; MARIELA ROLDÁN OLARTE; ROSA MARÍA SUSANA ALVAREZ
Revista:
CHEMISTRY SELECT
Editorial:
Wiley Online Library
Referencias:
Año: 2019 vol. 4 p. 3706 - 3716
ISSN:
2365-6549
Resumen:
A detailed biochemical characterization of the ooplasm wascarried out after elimination of the interference of theglycoproteins of the zona pellucida by both spectral subtractionand enzymatic degradation. The spectra thus obtainedallowed the identification of new spectral markers suitable todistinguish between immature and in vitro matured oocytes aswell as the observation of several weak bands correspondingto the less abundant components such as carbohydrates andpolynucleotides. This is the first study by Raman spectroscopyof bovine oocytes without the zona pellucida and constitutesthe basis for future assessments of structural/maturationaldeficiencies that could compromise the success of fertilization.
