Genistein affects proliferation and migration of bovine oviductal epithellial cells

GARCÍA, D.C; VALDECANTOS, PA.; MICELI, D.C; MARIELA ROLDÁN OLARTE

RESEARCH IN VETERINARY SCIENCE

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2017

0034-5288

Genistein is one of the most abundant isoflavone in soybeans. This molecule induces cell cycle arrest and apoptosis in different normal and cancer cells. Genistein has been of considerable interest due to the adverse effects on bovine reproduction, altering the estrous cycle, implantation and fetal development and producing subfertility or infertility. The objective of this work was to study the effects of genistein in the expression of selected genes involved in the regulation of cell cycle and apoptosis. Primary cultures of bovine oviductal epithelial cells (BOEC) were treated with different genistein concentrations (0.2, 2 and 10 μM) for analyzing CYCLIN B1, BCL-2 and BAX gene expression by Real-time RT-PCR. Results showed that genistein down-regulated CYCLIN B1 expression, affecting the cell cycle progression, and decreased the BCL-2/BAX ratio starting from 2 μM of genistein. In addition, to test if genistein affects the migration of the BOEC, in vitro wound healing assays were performed. A significant reduction in cell migration after 12 h of culture was observed at both 0.2 and 10 μM genistein concentrations. Also, in presence of genistein the percentage of mitotic cells decreased, nevertheless apoptotic cells percentages were not affected. These findings indicate that genistein has an inhibitory effect on the proliferation and migration of BOEC, suggesting that dietary genistein could influence the normal physiology of the oviductal epithelium.