INVESTIGADORES
PESCARETTI Maria De Las Mercedes
congresos y reuniones científicas
Título:
DEVELOPMENT AND OPTIMIZATION OF AN ATTENUATED VACCINE DESIGNED TO CONTROL Salmonella INFECTION
Autor/es:
MARIELA A. TORRES; MÓNICA F. TORREZ LAMBERTI; JUAN VICENTE FARIZANO; MARÍA DE LAS MERCEDES PESCARETTI; CARLOS J MINHAK; MÓNICA A. DELGADO
Reunión:
Congreso; Congreso de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2013
Resumen:
Foodborne diseases (FBD) are highly related to the low income
areas and represent one of the main problems worldwide. The most
common causative agent of FBD is Salmonella. The process of
Salmonella infection to non-phagocytic cells (epithelial cells)
involves the adhesion, invasion, maturation of Salmonella-containing
vacuoles and replication steps. The RcsCDB regulatory system is
deeply involved in controlling the expression of genes required to
establish these stages. In our laboratory, we have obtained the rcsC11
attenuated mutant, affecting the RcsC sensor, which causes
constitutive activation of the system. In this study we evaluated
the ability of both the wild-type strain and the rcsC11 mutant of S.
Typhimurium to infect epithelial cells (non-phagocytic) and
macrophage cells (phagocytes). We determined that the rcsC11
attenuated mutant was capable of producing a strong immunogenic
response. Afterward, the mutant was gradually removed because it
was unable to replicate in macrophages, in contrast to other well
described Salmonella attenuated mutants. Furthermore, we
constructed the pMAT13 plasmid vector that was able to replicate
in S. Typhimurium. The rcsC11 mutant transformed whit pMAT13
were able to express and secrete heterologous proteins. This finding
indicates that the rcsC11 mutant is a good candidate for the
development of new vector vaccines.