Complete nucleotide sequence of Alfalfa mosaic virus isolated from alfalfa (Medicago sativa L.) in Argentina

TRUCCO VERONICA; DE BREUIL SOLEDAD; BEJERMAN, NICOLÁS; LENARDON, SERGIO; GIOLITTI, FABIÁN

VIRUS GENES

SPRINGER

Lugar: Berlin; Año: 2014 vol. 48 p. 562 - 565

0920-8569

The complete nucleotide sequence of an Alfalfamosaic virus (AMV) isolate infecting alfalfa (Medicagosativa L.) in Argentina, AMV-Arg, was determined. Thevirus genome has the typical organization described forAMV, and comprises 3,643, 2,593, and 2,038 nucleotidesfor RNA1, 2 and 3, respectively. The whole genomesequence and each encoding region were compared withthose of other four isolates that have been completelysequenced from China, Italy, Spain and USA. The nucleotideidentity percentages ranged from 95.9 to 99.1 % forthe three RNAs and from 93.7 to 99 % for the protein 1(P1), protein 2 (P2), movement protein and coat protein(CP) encoding regions, whereas the amino acid identitypercentages of these proteins ranged from 93.4 to 99.5 %,the lowest value corresponding to P2. CP sequences ofAMV-Arg were compared with those of other 25 availableisolates, and the phylogenetic analysis based on the CPgene was carried out. The highest percentage of nucleotidesequence identity of the CP gene was 98.3 % with a Chineseisolate and 98.6 % at the amino acid level with fourisolates, two from Italy, one from Brazil and the remainingone from China. The phylogenetic analysis showed thatAMV-Arg is closely related to subgroup I of AMV isolates.To our knowledge, this is the first report of a completenucleotide sequence of AMV from South America and thefirst worldwide report of complete nucleotide sequence ofAMV isolated from alfalfa as natural host.