Evidence for the involvement of Lfc and Tctex-1 in axon formation

CONDE, CECILIA; ARIAS, CRISTINA; ROBIN, MARIA; LI, AIQUN; SAITO, MASAKI; CHUANG, JEN-ZEN; NAIRN, ANGUS C.; SUNG, CHING-HWA; CÁCERES, ALFREDO

JOURNAL OF NEUROSCIENCE

SOC NEUROSCIENCE

Año: 2010 vol. 30 p. 6793 - 6800

0270-6474

RhoA and Rac play key and opposite roles during neuronal polarization. We now show that Lfc, a guanosine nucleotide exchange factor (GEF), localizes to the Golgi apparatus and growth cones of developing neurons and negatively regulates neurite sprouting and axon formation through a Rho signaling pathway. Tctex-1, a dynein light chain implicated in axon outgrowth by modulating actin dynamics and Rac activity, colocalizes and physically interacts with Lfc, thus inhibiting its GEF activity, decreasing Rho-GTP levels, and functionally antagonizing Lfc during neurite formation. Copyright © 2010 the authors.