Tomato second cycle hybrids as source of genetic variability for fruit quality traits

PEREIRA DA COSTA, J. H.; RODRÍGUEZ, G.R.; LIBERATTI, D.R.; MAHUAD, S.L.; MARCHIONNI BASTÉ, E.; PICARDI, L.A.; ZORZOLI, R; PRATTA, G.R.

CROP BREEDING AND APPLIED BIOTECHNOLOGY

BRAZILIAN SOC PLANT BREEDING

Año: 2016

1518-7853

The aim was to study the phenotypic and molecular variability generated in a tomato F2 generation derived from a SCH (Second Cycle Hybrid) in order to detect QTLs for some fruit traits. Different genome coverage was achieved by three types of molecular markers (polypeptides, SRAP and AFLP). Different degrees of polymorphism were detected by SRAP and AFLP at the DNA structure level and also by polypeptides at the DNA expression level. The first two markers showed associations with phenotype variation and were able to detect QTLs involved in important agronomic traits such as fruit shelf life, soluble solids content, pH and titratable acidity. New gene blocks originated by recombination during the first cycle of cross were detected. This work confirmed that the observed phenotypic differences correspond to a new gene rearrangement and that these new gene blocks are responsible for the presence of the observed genetic variability for these traits.