Biological responses induced by high molecular weight chitosan administrated jointly with Platelet-derived Growth Factors in different mammalian cell lines

DI SANTO, MARIANA CAROLINA; ALAIMO, AGUSTINA; ACEBEDO, SOFÍA LORENA; SPAGNUOLO, CARLA; POZNER, ROBERTO; PÉREZ, OSCAR EDGARDO

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2020 vol. 158 p. 953 - 967

0141-8130

In thiswork,we studied cellular responses known to be involved in tissue regeneration, such as proliferation,migration and tubulogenesis under High Molecular Weight Chitosan (HMWC) and recombinant Platelet-derived Growth Factor (PDGF) treatments using an in vitro cell culture approach. Wealso analysed changes inmitochondrial dynamics that could be associatedwith such biological responses. For this proposes, endothelial human cell lines (EA.hy926 and ECFC) and 3T3-L1mouse fibroblastswere used. The intracellular uptake of HMWCand theirco-localization with acidic compartments were evaluated. Our results show that HMWC enhance PDGF-induced proliferation and cell migration in 3T3-L1 fibroblasts. An increase in PDGF-induced mitochondrial fragmentation was observed in 3T3-L1 cell line, but not in EA.hy926 cells, after the addition of HMWC. Endothelial cells, EA. hy926 and ECFC, potentiate their tubulogenesis capacity with the only addition of HMWC. The HMWC/PDGFBB treatment notably enhanced tubule formation showing a synergistic effect when act combined in cell culture medium. The knowledge of these cellular responses can be used to design new tissue repair strategies using HMWC and PDGF.