Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins.

CARLA C. SPAGNUOLO; RUDOLPH J. VERMEIJ; ELIZABETH JARES-ERIJMAN

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

American Chemical Society

Lugar: Washington; Año: 2006 vol. 128 p. 12040 - 12041

0002-7863

We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensitivity, higher absorbance and quantum yield than FlAsH, and F4FlAsH adds a new color to the palette of biarsenical dyes. The two probes also provide a new FRET pair with a larger Ro value (54 Å) than any previously obtained with biarsenical dyes.