EFFECT OF LIVER OVEREXPRESSION OF BRECEPT ON A RAT MODEL OF NAFLD

CÁMARA CA; ANABELA LA COLLA; TANIA MELINA RODRÍGUEZ; DEWEY RA; ANDREA N CHISARI

Mar del Plata

Congreso; LXIII Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2020

Sociedad Argentina de Investigación Clínica

Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disorder in developed countries, affecting up to 20% of the global population. Treatments for NAFLD patients are limited. Thus, the development of treatments to improve NAFLD patient?s health, is of clinical relevance. TGF-β pathway is related to hepatic steatosis and fibrogenesis in NAFLD. We have described a new soluble variant of TGF-β type II receptor, called TGFBR2-SE, that was fused to the Fc portion of human IgG (TGFBR2-SE/Fc), leading to the development of Brecept (Br). The aim of this work was to study, in a high fat high sucrose diet (HFHSD)-induced NAFLD rat model, the effect of lentiviral-mediated liver overexpression of Br (Lv-Br). We compared three experimental groups: Control, HF-HSD, and HFHSD+Br that received an intrahepatic injection of Lv-Br at week 17. Before Lv-Br injection, we observed insulin resistance in the HFHSD group (p