Comparison of antimicrobial activity and stability study of doxycycline hyclate and its inclusion complex development of a new powerful compound

KOGAWA, A.C.; ZOPPI, A.; QUEVEDO, M.A.; LONGHI, M.R.; NUNES SALGADO, H.R.

Araraquara

Congreso; II International symposium on drug discovery; 2011

Comité Organizador del II International symposium on drug discovery

Doxycycline hyclate (DOX), a broad-spectrum antibiotic, is part of the Unified Health System in Brazil, which is free-distributed to all by prescription. Unfortunately 1DOX is quite susceptible to light. 2A commonly applied approach to increase the stability of drugs is the formation of complexes with β-cyclodextrin (βCD). Despite the growing number of studies using cyclodextrins (CDs) in the formation of IC, this approach would not work to isolate the drug from the medium in which it is placed, but the external environment, because of the characteristic of DOX to be photosensitive and thus to compare their antimicrobial activity. After thermal and spectroscopic analysis to confirming the inclusion of DOX in βCD and showed how it is complexed, we carried out the development and validation of microbiological turbidimetric method for the quantification of DOX. The aim of this work was to compare, using the turbidimetric microbiological method, both the antimicrobial activity of the IC with DOX as the antimicrobial activity between them after exposure to UV light.