INVESTIGADORES
QUEVEDO Mario Alfredo
artículos
Título:
Quantitative plasma determination of a novel antiretroviral derivative of zidovudine by solid-phase extraction and high-performance liquid chromatography
Autor/es:
QUEVEDO, M.A.; TEIJEIRO, S.A.; BRINÓN, M.C.
Revista:
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Editorial:
Springer
Referencias:
Lugar: Heidelberg, Alemania; Año: 2006 vol. 385 p. 377 - 384
ISSN:
1618-2642
Resumen:
A solid-phase extraction methodology, followed by high-performance liquid chromatography (HPLC) quantification with UV absorbance detection (lambda = 267 nm), was developed in order to study the stability of 3'-azido-3'-deoxy-5'-O-isonicotinoylthymidine (AZT-Iso), a novel derivative of the antiretroviral AZT, in different matrixes. The half-lives (t1/2) for AZT-Iso were 1.19, 1.13 and 0.30 h for human, rat and rabbit plasma, respectively, and 14.91 and 25.49 h for potassium phosphate buffer (pH 7.4) and human serum albumin solution, respectively. The HPLC method proved to be selective, sensitive and accurate. Good recovery, linearity and precision were achieved using p-fluorophenol as an internal standard. The validity of this method was tested using synthetic mixtures of the intact drug with its decomposition products. In conclusion, the method presented is applicable to in vivo pharmacokinetics studies of AZT-Iso in rats.
