Tyro3 is upregulated in dendritic cells and macrophages exposed to extracellular vesicles from E. multilocularis.

ORTIZ WILCZYÑSKI; NICOLAO MA. CELESTE; RODRIGUEZ R CHRISTIAN; CUMINO ANDREA C; CARRERA SILVA ANTONIO EUGENIO

Mar del Plata

Congreso; LXX Reunión anual de la Sociedad Argentina de Inmunología (SAI); 2022

Sociedad Argentina de Investigaciones Clínica y Sociedades Conjuntas

The Echinococcus multilocularis (Em) cestode maintains intense communication with its host employing, among others, EVs as carriers to transport functional proteins, lipids, RNAs and other metabolites conditioning host immune response. Here, we explored the influence of EVs on the programing and differentiation of human antigen presenting cells (APCs). Blood CD14+ monocytes were isolated and differentiated toward Macrophages (Mø) and dendritic cells (DCs) under EVs treatment purified from Em protoscoleces. The phenotype, activation state, and level of TYRO3, AXL and MERTK (TAM) receptors on these Mø and DCs were analyzed by flow cytometry, qPCR, and with functional assays. EVs conditioning treatment of DCs resulted in upregulation of the integrin CD11c, and double positive HLA-DR with costimulatory molecules CD40, CD80, CD86 cells (p