GIULIETTI Ana Maria
congresos y reuniones científicas
Isolation and characterization of glyphosate degrading bacteria for rhizoremediation strategies
MASSOT F.; OSTRERA, JM; VASQUEZ, S; BAILLARES, M; GIULIETTI, AM; MERINI, LJ
Mar del Plata
Congreso; LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2015
The Humid Pampa is the main agricultural region inArgentina and one of the most important crop fields in South America, covering about52 million ha. As part of the modern agriculture practices, more than 300,000tons of pesticides are applied every year, were about 65% are formulations ofthe herbicide glyphosate (N?phosphonomethylglicine). In order to develop anefficient, eco-friendly, solar driven system to mitigate the environmental impactof glyphosate use in the agricultural exploitation context, the aim of thiswork is to find and characterize glyphosate degrading bacteria able to beassociated to Lotus spp. roots. Thisplant-bacteria system will be use to enhance the glyphosate degradation in therhizosphere influence zone and reduce its residues in agricultural soils andrunning off waters. To achieve this objective, bulk and Lotus rhizospheric soil samples were collected from both,glyphosate treated and control pasture plots. Then, batch liquid cultureswere performed using minimum salt enrichment media with glyphosate as the solecarbon or phosphorus source respectively. Among isolates, twoglyphosate-degrading strains were selected and culturally/genotipicallycharacterized. Glyphosate degradation in culture media was determined by FMOC-Clderivatization followed by UPLC. In addition, plant growth promotion capabilitieswere also determined for both strains.