GIULIETTI Ana Maria
congresos y reuniones científicas
A.tumefaciens and biobalistic mediated transformation of Morinda citrifolia cell suspensions
QUEVEDO, C; GIULIETTI, A. M.; RODRIGUEZ TALOU, J.
Mar del Plata
Congreso; XLIII Reunion Anual - SAIB; 2007
A. TUMEFACIEN SAND BIOLISTIC MEDIATED TRANSFORMATION OF MORINDACITRIFOLLIA CELL SUSPENSIONS Quevedo CV, GiuliettiAM, Rodríguez Talou J. Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, UBA,Argentina. E-mail: email@example.com M. citrifollia accumulate anthraquinones (AQs) which are secondary metabolites with therapeutic potential. AQs are formed from the methylerythritol 4-phosphate (MEP) pathway. 1-deoxy- D-Xylulose-5-phosphate synthase (DXS) the first enzyme in the MEP pathway catalyzes the transketolase reaction converting pyruvate and glyceraldehyde 3-phosphate into 1-deoxy-DXylulose- 5-phosphate. Also it has been shown that Octadecanoid- Derivative Responsive Catharanthus Apetala 2-domain Protein transcription factors (ORCA) induce the synthesis of specific enzymes from secondary metabolism as well as another kind of response defenses against pathogens. In the present work we used to different methods to obtain transgenic cell lines of . mediated transformation and biolistic. Two binary vectors were used, in the first one the cDNA of obtained from , was cloned between the Cauliflower mosaic virus 35S-promoter (CaMV-35S) and the potato proteinase inhibitor terminator (Pit). The cassette expression was inserted into the binary vector pMOG22-GUS, containing the GUS reporter gene. In the second one, the ORCA gene was cloned into the pCAMBIA 1300binary vector containing GFP as a reporter gene. cell suspensions were transformed by and biolistic. Transformations were checked by expression of the reporter genes, GUSandGFP