CONTRATADOS
GIULIETTI Ana Maria
artículos
Título:
Exploring different strategies to express Dengue virus envelope protein in a plant system
Autor/es:
MART├ŹNEZ, C; TOPAL, E; GIULIETTI A M; RODRIGUEZ TALOU, J; MASON, H
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
SPRINGER
Referencias:
Año: 2010 vol. 32 p. 867 - 867
ISSN:
0141-5492
Resumen:
Abstract Dengue virus envelope glycoprotein (E-protein) is themain protein associatedwith immunity induction. To produce a candidate for subunit vaccines and to provide an antigen for diagnostic kits, it was expressed in a novel plant system using deconstructed viral modules. A truncated version of the E-protein was designed to be expressed alone and co-expressed with Dengue virus structural proteins. As well, the critical domain III of E-protein was fused to hepatitis B core antigen (HBcore). The recombinant proteins were produced in Nicotiana benthamiana plants and were reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. (E-protein) is themain protein associatedwith immunity induction. To produce a candidate for subunit vaccines and to provide an antigen for diagnostic kits, it was expressed in a novel plant system using deconstructed viral modules. A truncated version of the E-protein was designed to be expressed alone and co-expressed with Dengue virus structural proteins. As well, the critical domain III of E-protein was fused to hepatitis B core antigen (HBcore). The recombinant proteins were produced in Nicotiana benthamiana plants and were reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. (E-protein) is themain protein associatedwith immunity induction. To produce a candidate for subunit vaccines and to provide an antigen for diagnostic kits, it was expressed in a novel plant system using deconstructed viral modules. A truncated version of the E-protein was designed to be expressed alone and co-expressed with Dengue virus structural proteins. As well, the critical domain III of E-protein was fused to hepatitis B core antigen (HBcore). The recombinant proteins were produced in Nicotiana benthamiana plants and were reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. (E-protein) is themain protein associatedwith immunity induction. To produce a candidate for subunit vaccines and to provide an antigen for diagnostic kits, it was expressed in a novel plant system using deconstructed viral modules. A truncated version of the E-protein was designed to be expressed alone and co-expressed with Dengue virus structural proteins. As well, the critical domain III of E-protein was fused to hepatitis B core antigen (HBcore). The recombinant proteins were produced in Nicotiana benthamiana plants and were reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. Dengue virus envelope glycoprotein (E-protein) is themain protein associatedwith immunity induction. To produce a candidate for subunit vaccines and to provide an antigen for diagnostic kits, it was expressed in a novel plant system using deconstructed viral modules. A truncated version of the E-protein was designed to be expressed alone and co-expressed with Dengue virus structural proteins. As well, the critical domain III of E-protein was fused to hepatitis B core antigen (HBcore). The recombinant proteins were produced in Nicotiana benthamiana plants and were reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies. Nicotiana benthamiana plants and were reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies.