GIULIETTI Ana Maria
congresos y reuniones científicas
Hyoscyamine 6--hydroxylase, a recombinant biocatalyst for the industrial productionof scopolamine
CARDILLO, A.; RODRIGUEZ TALOU, J.; GIULIETTI A. M.
Simposio; IBS2008; 2008
Hyoscyamine and scopolamine are tropane alkaloids widely used as pharmaceuticals due to their anticholinergic activity. Scopolamine is the more valuable, with a demand 10 times higher than that of hyoscyamine [1, 2] . Brugmansia candida (syn Datura candida) is a South American native plant and a tropane alkaloid producer . The conversion of hyoscyamine into scopolamine and 6b-hydroxyhyoxcyamine (anisodamine) is carried out by Hyoscyamine 6-b hydroxylase (H6H, EC 18.104.22.168). The development of a recombinant cell harbouring the plant enzyme constitutes an interesting and useful strategy for performing pharmaceutical processes This work reports the functional expression of H6H enzyme in Saccharomyces cerevisiae as a potential tool for the industrial production of scopolamine