Effect of ferrous sulfate and vitamin E on preventive supplementation of anemia during early childhood: an in vitro model

GAMBARO R; SEOANE A; PADULA G

BRITISH JOURNAL OF NUTRITION

CAMBRIDGE UNIV PRESS

Lugar: Cambridge; Año: 2022 vol. 20 p. 1 - 10

0007-1145

Iron deficiency is the leading cause of anemia. In Argentina, the prevalence of anemia andiron deficiency is very high; for that reason, the Argentine Society of Pediatrics recommendsdaily ferrous sulfate supplementation as a preventive treatment strategy. Alternatively,weekly ferrous sulfate supplementation has also been shown to be effective for anemiaprevention. Excess iron could be related to oxidative stress, which may in turn causecytomolecular damage. Both can be prevented with vitamin E supplementation. We evaluatedthe effect of both daily and weekly ferrous sulfate supplementation combined with two dosesof vitamin E on cell viability, oxidative stress and cytomolecular damage in peripheral bloodcultured in vitro. The experimental design included the following groups: untreated negativecontrol, two vitamin E controls (8.3 and 16.6 μg/ml), weekly ferrous sulfate supplementation(0.55 mg/ml) with each vitamin E dose, daily ferrous sulfate supplementation (0.14 mg/ml)with each vitamin E dose, and a positive control. Daily ferrous sulfate supplementationdecreased cell viability and increased the levels of reactive oxygen species, lipid peroxidationand cytomolecular damage (P