Doramectin induced cytotoxic and genotoxic effects on bovine peripheral lymphocytes and cumulus cells in vitro

ANCHORDOQUY JM; ANCHORDOQUY JP; NIKOLOFF N; GAMBARO R; PADULA G; SEOANE A; FURNUS C

JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH. PART B - PESTICIDES, FOOD CONTAMINANTS, AND AGRICULTURAL WASTES

TAYLOR & FRANCIS INC

Lugar: Londres; Año: 2019

0360-1234

The effect of doramectin (DOR) was tested on two experimental somatic bovine cells in vitro:peripheral lymphocytes (PL) and cumulus cells (CC). The cytotoxicity and genotoxicity of DORwere assessed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay,single cell gel electrophoresis assay (SCGE) and cytokinesis-block micronucleus cytome (CBMN Cyt)assay. Both cells were treated with three concentrations of DOR (20, 40, 60 ngmL?1) for 24 h. Theresults obtained from PL demonstrated that DOR was able to induce cytotoxic effect and DNAdamage with all concentrations tested. Additionally, DOR increased micronuclei (MNi) frequencyand nuclear buds (NBuds) with 20, 40, 60 ngmL?1, and nucleoplasmic bridges (NPBs) only with40 ngmL?1. On the other hand, the three concentrations of DOR were not able to induce cytotoxiceffect and DNA damage using SCGE in the bovine CC. Nevertheless, the two higher concentrationsof DOR (20, 40 mg mL?1) significantly increased the frequency of micronucleus formation in bovineCC. These results represent the first experimental evidence of genotoxic and cytotoxic effectsexerted by DOR on bovine PL and CC.