Cytotoxic and genotoxic effects induced by enrofloxacin in two experimental models of bovine cells in vitro: peripheral lymphocytes and cumulus cells.

ANCHORDOQUY JP; ANCHORDOQUY JM; NIKOLOFF N; GAMBARO R; PADULA G; FURNUS C; SEOANE AI

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

SPRINGER HEIDELBERG

Lugar: Heidelberg; Año: 2019 vol. 26 p. 2998 - 3005

0944-1344

The in vitro effect of enrofloxacin (EFZ) was tested on two experimental somatic bovine cells in vitro: peripheral lymphocytes(PLs) and cumulus cells (CCs). The cytotoxicity and genotoxicity of this veterinary antibiotic were assessed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays, single-cell gel electrophoresis (SCGE) assay, andcytokinesis-block micronucleus cytome (CBMN cyt) assay. Cells were treated during 24 h, and three concentrations were tested(50 μg/mL, 100 μg/mL, 150 μg/mL).When EFZ was tested in PLs, the results demonstrated that the antibiotic was able to inducecell death and DNA damage with all concentrations. In addition, 50 μg/mL and 100 μg/mL EFZ increased frequencies ofmicronuclei (MNi). On the other hand, the highest EFZ concentration occasioned cellular cytotoxicity in CCs as evidenced bymitochondrial activity alterations. Nevertheless, EFZ was not able to induce DNA damage and MNi in CCs. These resultsrepresent the first experimental evidence of genotoxic and cytotoxic effects exerted by EFZ in bovine PLs and CCs.