Evaluation of histological techniques for the detection of fungal infections caused by Leptolegnia chapmanii (Oomycetes: Saprolegniales) in Aedes aegypti (Diptera: Culicidae) larvae.

DIKGOLZ, V.E.; TOLEDO, A. V.; TOPA, P.E.; LÓPEZ LASTRA, C.C.

FOLIA MICROBIOLOGICA

Myris Trade Ltd.

Lugar: Praga; Año: 2005 vol. 50 p. 125 - 127

0015-5632

We evaluated which of the fixatives and stains most frequently used for observations of insect tissues were most appropiated for histopathological visualization of entomopathogenic fungal infections with Leptolegnia chapmanii in larvae of Aedes aegypti. The best contrast between the host tissues and the fungal structures was obtained when using a combination of Carnoy fixative with Grocott staining contrasted with light green. Masson trichromic stain combined with 10% formaldehyde-phosphate buffer also provided satisfactory results ? a good contrast and clearly distinguishable host tissues and fungal structures.