STUDY OF ANTIGEN-FREE IMMUNOSTIMULANTING COMPLEXES IN A MURINE MODEL

SATLER, FLORENCIA; REIDEL, IVANA; FLOR, NOELIA; GARCIA, MARIA INES; DIEZ, CRISTINA; SIANO, ALVARO; HUMPOLA, MARÍA VERONICA; VEAUTE, CAROLINA

Mar del Plata

Simposio; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

The non-specific stimulation of the immune system is an interestingstrategy for the prophylaxis and early treatment of infections in circumstanceswhere there is a lack of specific treatments such as vaccinesavailable. This work aimed to evaluate different formulationsas non-specific immunostimulants in a murine model. The formulationswere based on an ODN-CpG combined with 4 gemini-type lipopeptides: AG2-C16, AG2-C18, AG10-C16 and AG10-C18. Theoptimal gemini:ODN-CpG ratio to achieve the ODN-CpG encapsulationwas determined using agarose electrophoresis. The immunostimulatorycapacity was studied in vitro and in vivo by qPCR.Total splenocytes collected from BALB/c mice, without prior treatment,were stimulated in vitro with the formulations or the individualcomponents. No stimulation was used as basal condition (control).Formulations based on AG2-C16 were evaluated in vivo using BALB/c mice immunized with 3 doses. Control group was immunizedwith buffer. Lymph nodes were harvested 72hs after last dose forRNA purification. In both studies, the expression levels of thesegenes were measured: IFN-γ, TNF-α, IL-6 and CD-80. Resultswere expressed as ΔΔCT compared to the corresponding control,using GAPDH as housekeeping