NEW APPROACHES OF PHOTODYNAMIC THERAPY AGAINST GLIOBLASTOMA: ASSAYING METALLETED PORPHYRIN DOPED CONJUGATED POLYMER NANOPARTICLES AND CELLULAR DELIVERY IN 3D CULTURE MODEL

LUIS EXEQUIEL IBARRA; LUCÍA BEAUGÉ; GABRIELA VALERIA PORCAL; RAMIRO MARTIN SPADA; CARLOS ALBERTO CHESTA; RODRIGO EMILIANO PALACIOS; VIVIANA ALICIA RIVAROLA

Bs As

Congreso; Reunión Conjunta de Sociedades de BioCiencias; 2017

A new field of Photodynamic Therapy (PDT) is the treatment of brain tumors,such as Glioblastoma; and the study of a wide range of photosensitizers (PS) aswell as delivery systems to arrive to this tumor are of particular importance.OBJETIVES Evaluate PDT effect of CPNs in U87 MG glioblastoma cells using 2D and3D culture models. Evaluate biocompatibility and cargo uptake of CPNs in mononuclearcells (monocytes and macrophages). Assess the homing ability of mononuclear cells into glioblastomaspheroids previously loaded with CPNs.Conjugated polymer nanoparticles (CPNs) have excellent properties that make them suitable as photosensitizers (PS). In this work, we propose to study a strategy of cell vectorization of CPNs into Glioblastoma spheroids in an attempt to improve PDF efficacy. This novel strategy employs macrophages recruited in the tumor, which previously are intended to be preloaded with CPNs based on Poly(9,9-dioctylfluorenealtbenzothiadiazole) (F8BT) and platinum octaethylporphyrin (PtOEP).