Enhanced anti-proliferative effect in melanoma cells by catalase-magnetite nanocomplex

S. MUNICOY; I. L. IBAÑEZ; H. DURAN; M. G. BELLINO

Congreso; VIII Meeting of the Society for Free Radical Biology and Medicine; 2013

Society for Free Radical Biolog y and Medicine

  Catalase, an enzyme degrading hydrogen peroxide, has been proposed as an anti-proliferative agent for tumor cells. The development of versatile catalase-magnetic nanocomplex remain challenging. Here we evaluate magnetite nanoparticles (MNPs) functionalized with catalase (MNP-CAT) in tumor cell proliferation. We synthetized MNPs by co-precipitation and mixed them with catalase; and human melanoma cells were incubated with CAT, MNPs or MNP-CAT. The MNPs stability was analyzed by Dynamic Light Scattering, catalase activity was determined spectrophotometrically and cell proliferation was evaluated by MTT assay. MNP-CAT exhibited high stability along time as compare with MNPs and catalase activity was well-preserved in MNP-CAT up to 42°C and in range of pH 3-9. A significant decrease of tumor cell proliferation was observed in presence of MNP-CAT as compared with CAT treatment while tumor cell proliferation was not affected by MNPs. Cells incubated with MNP-CAT and exposed to magnets in order to remove MNP-CAT reached similar levels of proliferation as compare with non-treated cells or cells treated with catalase that was removed by changing the medium. We conclude that MNPs are promising carriers for catalase delivery improving the antioxidant enzyme effect in tumor cells with the advantage that MNP-CAT can be easily removed by a magnetic field.