Immobilization of Mucor miehei lipase on titania mesoporous materials

Y. MARTINEZ; M. G. BELLINO; D. KAPLAN; G. J. A. A. SOLER-ILLIA; G. R. CASTRO

Boston

Congreso; 240th ACS National Meeting - Chemistry for Preventing & Combating; 2010

American Chemical Society

Mesoporous materials provide support and protection to confined biomolecules like enzymes under harsh environmental conditions. The strong interactions between the matrix surface and proteins reduce significantly the catalytic activity; thus, a coating with a "friendly" molecule, like BSA (Bovine Serum Albumin) is required. In this work, the effect of temperature and pH on the specific activity (SA) of lipase from Mucor Miehei inmobilized on titania (TiO2) mesoporous supports coated with BSA was studied. Mesoporous supports with a pore diameter of 10 nm (B), 5-50 nm (C) were used. Lipase SA was determined in non-mesoporous (NMPS) and mesoporous (MPS) supports at temperatures ranging from 37 to 52 ºC and in 3.0 -8.5 pH range. Significant differences in the SA were found between NMP and C MP at 45ºC and 47 ºC. This result indicates that the pore systems of C MPS provide a specific protection mechanism that prevents enzyme thermal denaturing.