Developmental toxicity of Bisphenol A diglycidyl ether (epoxide resin BADGE) during the early life cycle of a native amphibian species

HUTLER WOLKOWICZ I.; SVARTZ G.; ARONZON C.M; PERÉZ COLL C.S.

ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY

SETAC PRESS

Lugar: Buenos Aires; Año: 2016 vol. 35 p. 3031 - 3038

0730-7268

Bisphenol A diglycidyl ether (BADGE) is used in packaging materials, in epoxy adhesives, and as an additive for plastics, butit is also a potential industrial wastewater contaminant. The aim of the present study was to evaluate the adverse effects of BADGE onRhinella arenarum by means of standardized bioassays at embryo?larval development. The results showed that BADGE was more toxicto embryos than to larvae at all exposure times. At acute exposure, lethality rates of embryos exposed to concentrations of 0.0005 mg/LBADGE and greater were significantly higher than rates in the vehicle control, whereas lethality rates of larvae were significantly higherin concentrations of 10 mg/L BADGE and greater. The toxicity then increased significantly, with 96-h median lethal concentrations(LC50s) of 0.13 mg/L and 6.9 mg/L BADGE for embryos and larvae, respectively. By the end of the chronic period, the 336-h LC50swere 0.04 mg/L and 2.2 mg/L BADGE for embryos and larvae, respectively. This differential sensitivity was also ascertained by the 24-hpulse exposure experiments, in which embryos showed a stage-dependent toxicity, with blastula being the most sensitive stage andS.23 the most resistant. The most important sublethal effects in embryos were cell dissociation and delayed development, whereas themain abnormalities observed in larvae related to neurotoxicity, as scare response to stimuli and narcotic effect